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Potensi Streptomyces dalam Menghambat Escherichia coli Extended Spectrum Beta Lactamase Penyebab Urinary Tract Infection

Abstract

Introduction: Streptomyces can produce primer and secondary metabolites such as enzymes and antibiotics. The emergence of pathogens resistant to antibiotics causes increased deaths among sufferers. Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) is a pathogenic bacterium that causes urinary tract infections and is resistant to Beta-Lactam antibiotics. This research aimed to analyze the ability of Streptomyces isolated from soil around the Biology Study Program, Udayana University, to inhibit E. coli  ESBL.

Methods: Streptomyces were isolated from soil around the Biology Study Program, Faculty of Mathematics and Natural Sciences, Udayana University. Antagonist tests of Streptomyces isolates against Escherichia coli ESBL were done with dual culture. The Minimum Inhibition Concentration (MIC) of the Streptomyces isolate filtrate that inhibits E.coli ESBL was tested with the wheel diffusion test. The Streptomyces isolate that inhibits E.coli ESBL was identified with 16S rRNA.

Results: Four isolates of Streptomyces were isolated from soil, namely Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3 and Streptomyces sp 4. The Streptomyces sp.3 isolate can inhibit ESBL E. coli with an inhibition zone diameter of 16±0.1 mm and Minimum Inhibition Concentration (MIC) of 10% with an inhibition zone diameter of 20±0.13 mm. Molecular identification of Streptomyces sp.3 showed the homology with 5 isolates of Streptomyces in Genebank data; among them, Streptomyces rochei strain R-3B, Streptomyces enissocaesilis AL3, Streptomyces sp. SMVB 1, Streptomyces djakartensis and Streptomyces WAC627.

Conclusion: Streptomyces sp isolate 3 isolated from soil around the Biology Study Program Udayana University showed the highest inhibition of E. coli ESBL.

 

Latar Belakang: Streptomyces merupakan mikroorganisme yang mampu menghasilkan metabolit primer dan sekunder seperti enzim dan antibiotika. Munculnya bakteri patogen penyebab infeksi yang mengalami resistensi antibiotika meningkatkan resiko kematian terhadap penderita. Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) merupakan bakteri patogen penyebab Urinary Tract Infection (UTI) yang telah mengalami resistensi terhadap antibiotika golongan Beta-Lactam. Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces yang diisolasi dari tanah sekitar Program Studi Biologi, Universitas Udayana dalam menghambat pertumbuhan E. coli ESBL, mengatahui Minimum Inhibition Concentration filtrat Streptomyces terhadap E. coli  ESBL.

Metode: Streptomyces diisolasi dari tanah disekitar Program Studi Biologi, Fakultas Matematika dan Ilmu Pengatahuan Alam, Universitas Udayana. Uji antagonis isolat Streptomyces terhadap E. coli  ESBL dengan metode dual culture. Isolat Streptomyces yang menghambat E. coli  ESBL dilanjutkan uji Minimum Inhibition Concetration terhadap E. coli  ESBL dengan metode sumur difusi. Dilakukan identifikasi molekuler dengan 16S rRNA pada Streptomyces yang mampu menghambat E. coli.

Hasil: Diperoleh empat isolat Streptomyces dari tanah yaitu Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3 dan Streptomyces sp.4. Isolat Streptomyces sp.3 mampu menghambat E. coli ESBL dengan diameter zona hambat sebesar 16±0.1 mm dan Minimum Inhibition Concentration (MIC) 10% dengan diameter zona hambat sebesar 20±0.13 mm. Streptomyces sp.3 teridentifikasi secara molekuler mempunyai homologi dengan 5 spesies Streptomyces pada data genebank yaitu, Streptomyces rochei strain R-3B, Streptomyces enissocaesilis AL3, Streptomyces sp. SMVB 1, Streptomyces djakartensis dan Streptomyces WAC627.

Kesimpulan: Dari penelitian ini dapat disimpulkan bahwa Streptomyces yang diisolasi dari tanah mampu menghambat E. coli ESBL.

References

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How to Cite

Kartika, K. D., Made Pharmawati, Retno Kawuri, & Citra Gading. (2023). Potensi Streptomyces dalam Menghambat Escherichia coli Extended Spectrum Beta Lactamase Penyebab Urinary Tract Infection. Medicina, 54(3), 134–139. https://doi.org/10.15562/medicina.v54i3.966

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