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Variasi morfologi koloni burkholderia pseudomallei pada media ashdown

Abstract

Burkholderia pseudomallei is the causative agents of melioidosis. It is classified as “Category B†agent by the CDC due to the high fatality and it’s ability to mimics the symptoms of other diseases. This bacteria can survive and adapt in harsh environment and are resistent to various types of antibiotics. One of the adaptation that this bacteria have is the various types of colony morphologies and their ability to switch between types both in vitro and in vivo. One isolate of gram negative oxidase positive bacteria from Sanglah Hospital in Bali has been found to produce three types of colony morphology in McConkey media. It was confirmed by using PCR, Culture, and Gram Stain that those three different types of colonies were different types of colony morphologies that can be seen in Burkholderia pseudomallei. By using PCR, those three different types of colonies were identified as Burkholderia pseudomallei. Switching of colony morphology were also observed during subculturing from McConkey to Ashdown. From three different morphology in McConkey, only two different types of morphology found in Ashdown (mucoid and non-mucoid). Gram staining of those two types of colonies produce different bacterial morphology. Gram stain of mucoid colonies can be observed as regular gram negative rod bacteria while gram stain of non-mucoid colonies produce “bipolar staining†that are typical of Burkholderia pseudomallei. This showed that B. pseudomallei can exhibit different colony morphotypes and can undergo colony morphology switching in vivo.


Burkholderia pseudomallei merupakan bakteri yang menjadi penyebab melioidosis. Oleh CDC, bakteri ini dimasukkan sebagai “Category B†agent karena memiliki tingkat fatalitas yang tinggi serta sering meniru gejala penyakit lainnya. Bakteri ini memiliki ketahanan hidup yang tinggi serta dapat beradaptasi dengan lingkungan yang sulit. Salah satu adaptasi oleh bakteri ini adalah dalam perbedaan tipe koloni serta perubahan antar satu tipe ke tipe lainnya yang terjadi baik in vivo maupun in vitro. Pada penelitian ini ditemukan satu isolat bakteri gram negatif oxidase positif dari RSUP Sanglah di Bali yang menghasilkan tiga morfologi koloni di McConkey (MC). Pada penelitian ini, ditemukan bahwa ketiga tipe koloni tersebut merupakan koloni B. pseudomallei dengan morfologi yang berbeda menggunakan metode PCR, Kultur, dan Gram Staining. Ketiga koloni tersebut dipastikan sebagai B. pseudomallei saat dikonfirmasi menggunakan metode PCR. Selain itu, terlihat adanya perubahan morfologi koloni dari 3 tipe morfologi di McConkey menjadi 2 morfologi saat ditanam di media Ashdown (ASA). Kedua koloni di ASA tersebut memiliki morfologi non-mucoid dan mucoid. Pada pewarnaan Gram, Burkholderia pseudomallei dapat memberikan hasil pengecatan gram yang terlihat berbeda. Dibawah mikroskop, koloni dengan morfologi mucoid terlihat seperti bakteri batang gram negatif biasa sedangkan koloni dengan morfologi non-mucoid akan memperlihatkan “Bipolar Staining†yang khas dengan gambaran Burkholderia pseudomallei. Hal ini mengkonfirmasi bahwa B. pseudomallei dapat memiliki tipe morfologi koloni yang berbeda serta dapat merubah tipe morfologinya in vivo.

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How to Cite

PNC, S., NNS, B., & KJP, P. (2020). Variasi morfologi koloni burkholderia pseudomallei pada media ashdown. Medicina, 51(3). https://doi.org/10.15562/medicina.v51i3.964

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