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Perbedaan ekspresi matriks metalloproteinase-2 dan caspase-3 sel amnion pada persalinan preterm dengan ketuban pecah dini dan tanpa ketuban pecah dini

Abstract

Background: Premature rupture of membrane (PROM) is one of pregnancy complications that has become important medical issues all over the world including Indonesia, in regards to its prevalence, related morbidity and mortality not only for the mother but also for the fetus or neonate. The weakened amniotic membrane associated with increased degradation of extracellular matrix and apoptosis is an important factor underlying the pathogenesis of PROM. The increase of MMP-2 and caspase-3 expression is an important topic to study in order to understand the risk factors of PPROM.

Method: This is a cross sectional study involving 40 mothers with and without PROM with gestational age range from 20 to 37 weeks. The sample of amniotic membrane are taken consecutively from the edges of the broken site of amniotic membrane, sized 2 cm. The sample was then fixated within a phosphate buffer saline (PBS) solution. The sample was sent to Laboratorium Biomedik Terpadu, Faculty of Medicine Udayana University for MMP-2 and caspase-3 characterization using immunohistochemistry.

Result: Maternal age, gestational age, and BMI were not the risk factors of PROM. The expression of MMP-2 and caspase-3 were significantly higher in group with PROM as compared to those without PROM (p<0.05).

Conclusion: In conclusion, there were differences in the expression of MMP-2 and caspase-3 between group with PROM as compared to those without PROM.


Latar Belakang: Ketuban pecah dini (KPD) merupakan salah satu komplikasi kehamilan yang menjadi masalah kesehatan penting di seluruh dunia termasuk Indonesia, terkait dengan prevalensi, morbiditas dan mortalitas perinatal di samping maternal. Melemahnya selaput ketuban akibat degradasi komponen matriks ekstraseluler dan peningkatan apoptosis sel amnion merupakan faktor penting yang mendasari terjadinya KPD. Peningkatan kadar MMP-2 dan caspase-3 menarik untuk diteliti sebagai salah satu faktor risiko pada KPD preterm.

Metode: Penelitian ini merupakan penelitian potong lintang terhadap 40 orang ibu bersalin dengan dan tanpa KPD umur kehamilan 20-37 minggu. Sampel penelitian berupa jaringan amnion yang diambil secara consecutive sampling. Jaringan amnion diambil dari tepi robekan selaput ketuban berukuran lebar 2 cm setelah melahirkan, kemudian dimasukkan dalam larutan fiksasi phosphate buffer saline (PBS). Sampel selanjutnya dikirim ke Laboratorium Biomedik Terpadu FK Universitas Udayana Denpasar, untuk pemeriksaan ekspresi MMP-2 dan Caspase-3 menggunakan metode imunohistokimia.

Hasil: Umur, ibu usia gestasi dan IMT bukan merupakan faktor risiko KPD preterm. Ekspresi MMP-2 dan caspase-3 sel amnion pada KPD lebih tinggi secara bermakna dibanding ekspresi MMP-2 dan caspase-3 sel amnion tanpa KPD (p<0,05).

Kesimpulan: Penelitian ini menyimpulkan bahwa terdapat perbedaan ekspresi MMP-2 dan caspase-3 sel amnion pada kasus KPD dibanding tanpa KPD pada persalinan preterm.


References

  1. Getahun, D., Stricland, D., Ananth, C.,Fasset., Kirby., Jacobsen. Recurrent of Preterm Ruptured of Membranes In Relation to Interval Between Pregnancy. Am J Obstet and Ginecol. United States of America. 2012. Vol 220. pp570. El-6
  2. Soewarto, S. Ketuban Pecah Dini. Ilmu Kebidanan. Edisi Keempat. Jakarta: PT Bina Pustaka Sarwono Prawirohardjo. 2002. h. 677-682.
  3. Budijaya M, Negara KS. Profil Persalinan dengan Ketuban Pecah Dini di RSUP Sanglah Denpasar Periode 1 Januari – 31 Desember 2015. Denpasar: Program Pendidikan Dokter Spesialis-I Bagian/SMF Obstetri dan Ginekologi FK UNUD / RSUP Sanglah; 2016.
  4. Cunningham, F. G. Preterm Birth. Obstetri Williams 23rd. The McGraw-Hill Company, New York; 2010. 804-831.
  5. Menon, R, Fortunato, S. J. Distinct Pathophysiologic Pathways Induced by in vitro Infection and Cigarette Smoke in Normal Human Fetal Membranes. Am J Obstet Gynecol. 2009; 200:334.e1-334.e8.
  6. Mercer. Preterm Premature Rupture of Membranes. Am J Obst Gynecol. 2005; 101(1): 178-193.
  7. Liu X., Watkins K., Hussey., Luchok J., Amankra S. N. Effect of Maternal Stress on Low Birth Weight and Preterm Birth Outcomes Across Neighborhoods of South Carolina. J Maternal and Child Health. 2010; 14(2): 215-226.
  8. Abrantes, A. M., Casalta-Lopes, J., & Botelho, M. F. Biophysical Properties of Amniotic Membrane. In Amniotic Membrane. Springer Netherlands. 2015; 41-56.
  9. Saglam, A., Ozgur, C., Derwig, I., Unlu, B. S., Gode, F., and Mungan, T. The Role of Apoptosis in Preterm Premature Rupture of The Human Fetal Membranes. Archives of Gynecology and Obstetrics. 2013; 288(3): 501-505.
  10. Fortunato, S. J., Menon, R., Bryant, C. Programmed Cell Death (Apoptosis) as a Possible Pathway to Metalloproteinase Activation and Fetal Membrane Degradation in Premature Rupture of Membranes. Am J Obstet Gynecol. 2012; 182(6): 1468-76
  11. Rosai, J. Breast. In Rosai and Ackerman’s Surgical Pathology. 9th ed. Edinburg: Mosby; 2004. h. 1763-1876.
  12. Okeke T. C., Enwereji, J. O., Adiri, C. O., Morbidities, Concordance, And Predictor of Preterm Premature Rupture of Membranes Among Pregnant Women at the University of Nigeria Teaching Hospital Enugu, Nigeria. 2016. Vol 9 (6):737-741.
  13. Negara K., Suwiyoga K., Tunas K. Role of Caspase-3 as Risk Factors of Premature of Membrane. Biomed Pharmacol J 2017; 10 (4)
  14. Patil, S. & Patil, V. Maternal and Foetal Outcome in Premature Rupture of Membranes. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). 2014. 13(12): 56-81
  15. Ota, A., Yonemoto, H., Someya, A., Itoh, S., Kinoshita, K., Nagaoka, I. Changes in Matrix Metalloproteinase 2 Activities in Amniochorions During Premature Rupture of Membranes. J Soc Gynecol Investig. 2006; 13(8):592-97.
  16. Nagase H., Visse R., Murphy G. Structure and Functipn of Matrix Metalloproteinases and TIMPs. 2006; 68(3): 595-603
  17. Reuben, A., Trojanowska M., Znoyko, I. Collagen Binding Alpha 2beta1 and alpha1beta1 Integrin Play Contrasting Role in Regulation of Ets-1 Expression In Human Liver Myofibroblasts, A. Mol Cell Biochem 2006; 282: 89.
  18. McLaren, J., Malak, T. M., Bell, S. C. Structural Characteristics of Term Human Fetal Membranes Prior to Labour: Identification of An Area of Altered Morphology Overlying the Cervix. Human Reproduction. 1999; 14(1): 237–40
  19. Saglam, A., Ozgur, C., Derwig, I., Unlu, B.S., Gode, F., Mungan, T. The role of apoptosis in preterm premature rupture of the human fetal membranes. Arch Gynecol Obstet. 2013; 288:501-05.
  20. Brentnall, M., Rodriguez-Menocal, L., De Guevara, R. L., Cepero, E., and Boise, L. H. Caspase-9, caspase-3 and caspase-7 have Distinct Roles During Intrinsic Apoptosis. J BMC Cell Biology. 2013; 14 (1): 32.
  21. Reti, N. G., Lapas, M., Riley, C., Wlodek, M. E., Permezel, M., Walker, S., Rice, G.E. Why Do Membranes Rupture at Term? Evidence of Increased Cellular Apoptosis in the Supracervical Fetal Membranes. Am J Obstet Gynecol 2007; 196: 484.e1-10
  22. Klener, P., Andera L., Necas E., Zivny J. Cell Death Signalling Pathways in the Pathogenesis and Therapy of Haemotologic Malignancies. J Folia Biol. 2006; 52:(1-2).

How to Cite

Fatrisia, F., Negara, K. S., Suwiyoga, K., Suwardewa, T. G. A., Megadhana, I., Darmayasa, M., Putra, I. G. M., & Wiradnyana, A. A. G. P. (2020). Perbedaan ekspresi matriks metalloproteinase-2 dan caspase-3 sel amnion pada persalinan preterm dengan ketuban pecah dini dan tanpa ketuban pecah dini. Medicina, 51(2), 108–114. https://doi.org/10.15562/medicina.v51i2.887

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