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Pola kepekaan bakteri yang terisolasi dari kultur darah terhadap antibiotik di Unit Perawatan Intensif Anak RSUP Sanglah Denpasar tahun 2015-2016

Abstract

Pendahuluan: Resistensi antibiotik merupakan salah satu masalah global pada Unit Perawatan Intensif Anak (UPIA), termasuk di Bali. Patogen bakteri dan pola kepekaannya harus dipantau pada Rumah Sakit.

Metode: Penelitian deskriptif, retrospektif dari rekam medis dan register laboratorium Mikrobiologi Klinik RSUP Sanglah Denpasar, dari pasien usia 1 bulan – 12 tahun yang dirawat di UPIA RSUP Sanglah. Spesimen kultur darah dikumpulkan secara aseptik dari 2 sisi yang berbeda, dimasukkan dalam botol BacT/ALERT dan diinkubasi secara aerob. Tes kepekaan antibiotik dilakukan menggunakan automatic Vitek Compact 2 System (BiomeÌrieux). Data hasil kultur darah diambil dari register hasil kultur darah Laboratorium Mikrobiologi Klinik, dan data pasien diambil dari rekam medis UPIA RSUP Sanglah.

Hasil: Total sampel kultur darah berjumlah 510 didapatkan dari pasien yang dirawat di UPIA RSUP Sanglah selama waktu penelitian. Sebanyak 82 sampel dinyatakan positif, dimana 69,5% diantaranya merupakan bakteri gram positif. Mikroorganisme yang paling banyak ditemukan antara lain Staphylococcus hominis spp (21.9%), Staphylococcus epidermidis spp (12.1%), Staphylococcus haemolyticus ssp (8.5%), Salmonella spp (8.5%) dan Enterobacter cloacae (6%). Bakteri gram negatif masih sensitif terhadap Ampicillin, Cefepime, dan Ceftriaxone. Bakteri gram positif sensitif terhadap Gentamicin dan Linezolid.

Kesimpulan: Penelitian ini menunjukkan bahwa tidak semua antibiotik yang digunakan di UPIA dapat digunakan sebagai terapi empirik karena pola kepekaan bakteri yang berbeda. Penelitian ini menunjukkan bahwa terapi empirik di UPIA, termasuk di RSUP Sanglah Denpasar, harus berdasarkan pola kepekaan antibiotik lokal.


Introduction: Antibiotic resistance is a major worldwide problem in pediatric intensive care unit (PICU), including in Bali. The bacterial pathogens and their susceptibility patterns should be monitored in hospital settings.

Method: A descriptive, retrospective study from medical records and registers of Clinical Microbiology Laboratory, Sanglah Hospital Denpasar, taken from patients aged 1 month-12 years who were admitted in PICU of Sanglah Hospital. Specimens for blood cultures were collected aseptically from 2 different sites, inserted into BacT/ALERT bottles, and incubated aerobically. The bacterial identification and antibiotic susceptibility testing were performed using automatic Vitek Compact 2 System (BiomeÌrieux). Data of blood cultures results was taken from blood culture results registry of Clinical Microbiology Laboratory, and patient data was retrieved from medical records of PICU Sanglah Hospital.

Result: A total of 510-blood culture samples obtained from patients who admitted to PICU of Sanglah Hospital during the study period. Eighty-two samples (16.1%) were positive, which 69.5% of them were gram-positive bacteria. The most common microorganisms found were Staphylococcus hominis spp (21.9%), Staphylococcus epidermidis spp (12.1%), Staphylococcus haemolyticus ssp (8.5%), Salmonella spp (8.5%) and Enterobacter cloacae (6%). Most of gram-negative bacteria were still sensitive to Ampicillin, Cefepime, and Ceftriaxone. On the other hand, the gram-positive bacteria showed susceptible to Gentamicin and Linezolid.

Conclusion: This study shows that not all antibiotics used in the PICU can be used as empiric therapy because of differences of isolated bacteria susceptibility pattern. This study suggested that empirical therapy in PICU, including in Sanglah Hospital Denpasar, should be based on local antibiotics susceptibility pattern.


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How to Cite

Priskila, M., Wati, D. K., Suparyatha, I. B. G., Hartawan, I. N. B., Gustawan, I. W., Fatmawati, N. N. D., & Tunas, I. K. (2019). Pola kepekaan bakteri yang terisolasi dari kultur darah terhadap antibiotik di Unit Perawatan Intensif Anak RSUP Sanglah Denpasar tahun 2015-2016. Medicina, 50(3). https://doi.org/10.15562/medicina.v50i3.443

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